PrimePCR™ SYBR® Green Assay: SLC25A40, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049628

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042366
Assay Design:   exonic
Chromosome Location:   7:87464460-87464596question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000341119

Gene Information

SLC25A40 belongs to the SLC25 family of mitochondrial carrier proteins (Haitina et al. 2006 [PubMed 16949250]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC25A40
Gene Name:   solute carrier family 25, member 40
Aliases:   MCFP
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000075303
Entrez:   55972
UniGene:   Hs.208414
Chromosome Mapping:   7q21.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.400000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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