PrimePCR™ SYBR® Green Assay: ZNF638, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049627

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: ZNF638, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF638, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042365
Assay Design:   exonic
Chromosome Location:   2:71575975-71576077question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000410075 ENST00000264447 ENST00000409544 ENST00000455226 ENST00000454278 ENST00000437658 ENST00000417778 ENST00000454122 ENST00000544512 ENST00000355812 ENST00000377802

Gene Information

The protein encoded by this gene is a nucleoplasmic protein. It binds cytidine-rich sequences in double-stranded DNA. This protein has three types of domains: MH1 MH2 (repeated three times) and MH3. It is associated with packaging transferring or processing transcripts. Multiple alternatively spliced transcript variants have been found for this gene but the biological validity of some variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   ZNF638
Gene Name:   zinc finger protein 638
Aliases:   DKFZp686P1231, MGC26130, MGC90196, NP220, ZFML, Zfp638
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000075292
Entrez:   27332
Chromosome Mapping:   2p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.580000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download