PrimePCR™ SYBR® Green Assay: GRAMD4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049626

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042364
Assay Design:   exonic
Chromosome Location:   22:47022692-47022803question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000447351 ENST00000431155 ENST00000406902 ENST00000361034

Gene Information

GRAMD4 is a mitochondrial effector of E2F1 (MIM 189971)-induced apoptosis (Stanelle et al. 2005 [PubMed 15565177]).[supplied by OMIM Jan 2011]

Gene Symbol:   GRAMD4
Gene Name:   GRAM domain containing 4
Aliases:   DIP, KIAA0767, MGC149847, MGC90497, dA59H18.1, dJ439F8.1
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000075240
Entrez:   23151
UniGene:   Hs.475150
Chromosome Mapping:   22q13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.430000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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