PrimePCR™ SYBR® Green Assay: PTGS2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049604

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042341
Assay Design:   exonic
Chromosome Location:   1:186645124-186645271question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000367468

Gene Information

Prostaglandin-endoperoxide synthase (PTGS) also known as cyclooxygenase is the key enzyme in prostaglandin biosynthesis and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2 which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq Feb 2009]

Gene Symbol:   PTGS2
Gene Name:   prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Aliases:   COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000073756
Entrez:   5743
UniGene:   Hs.196384
Chromosome Mapping:   1q25.2-q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.680000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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