PrimePCR™ SYBR® Green Assay: DHRS9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049602

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042339
Assay Design:   exonic
Chromosome Location:   2:169952066-169952215question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000602501 ENST00000357546 ENST00000428522 ENST00000421653 ENST00000436483 ENST00000432060 ENST00000327239 ENST00000412271

Gene Information

Description Not Available

Gene Symbol:   DHRS9
Gene Name:   dehydrogenase/reductase (SDR family) member 9
Aliases:   3ALPHA-HSD, RDH15, RDHL, RDHTBE, RETSDR8, SDR9C4
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000073737
Entrez:   10170
UniGene:   Hs.179608
Chromosome Mapping:   2q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.970000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download