PrimePCR™ SYBR® Green Assay: HSD17B10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049585

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042322
Assay Design:   exonic
Chromosome Location:   X:53459261-53460683question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000357637 ENST00000375304 ENST00000168216 ENST00000375298 ENST00000254799 ENST00000499044 ENST00000502323 ENST00000514161 ENST00000545193 ENST00000439371 ENST00000294599 ENST00000356575 ENST00000271588 ENST00000367492 ENST00000232978 ENST00000377833 ENST00000375790 ENST00000419761 ENST00000595128 ENST00000601615 ENST00000600143

Gene Information

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids alcohols and steroids. The protein has been implicated in the development of Alzheimer's disease and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified but the full-length nature of only two transcript variants has been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   HSD17B10
Gene Name:   hydroxysteroid (17-beta) dehydrogenase 10
Aliases:   17b-HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1
RefSeq:   NC_000023.10 NT_011630.14 NG_008153.1
Ensembl:   ENSG00000072506
Entrez:   3028
UniGene:   Hs.171280
Chromosome Mapping:   Xp11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.600000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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