PrimePCR™ SYBR® Green Assay: RHOBTB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049583

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RHOBTB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RHOBTB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042320
Assay Design:   exonic
Chromosome Location:   10:62630324-62630459question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000337910 ENST00000357917

Gene Information

The protein encoded by this gene belongs to the Rho family of the small GTPase superfamily. It contains a GTPase domain a proline-rich region a tandem of 2 BTB (broad complex tramtrack and bric-a-brac) domains and a conserved C-terminal region. The protein plays a role in small GTPase-mediated signal transduction and the organization of the actin filament system. Alternate splicing results in multiple transcript variants. [provided by RefSeq Dec 2008]

Gene Symbol:   RHOBTB1
Gene Name:   Rho-related BTB domain containing 1
Aliases:   KIAA0740, MGC33059, MGC33841
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000072422
Entrez:   9886
Chromosome Mapping:   10q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.770000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download