This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0049583
PrimePCR™ PreAmp for SYBR® Green Assay: RHOBTB1, Human
PrimePCR™ Template for SYBR® Green Assay: RHOBTB1, Human
The protein encoded by this gene belongs to the Rho family of the small GTPase superfamily. It contains a GTPase domain a proline-rich region a tandem of 2 BTB (broad complex tramtrack and bric-a-brac) domains and a conserved C-terminal region. The protein plays a role in small GTPase-mediated signal transduction and the organization of the actin filament system. Alternate splicing results in multiple transcript variants. [provided by RefSeq Dec 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.