PrimePCR™ SYBR® Green Assay: ST6GALNAC2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049558

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042295
Assay Design:   exonic
Chromosome Location:   17:74568750-74569280question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000588920 ENST00000225276

Gene Information

ST6GALNAC2 belongs to a family of sialyltransferases that add sialic acids to the nonreducing ends of glycoconjugates. At the cell surface these modifications have roles in cell-cell and cell-substrate interactions bacterial adhesion and protein targeting (Samyn-Petit et al. 2000 [PubMed 10742600]).[supplied by OMIM Mar 2008]

Gene Symbol:   ST6GALNAC2
Gene Name:   ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2
Aliases:   FLJ45660, SAITL1, SIAT7, SIAT7B, SIATL1, ST6GalNAII, STHM
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000070731
Entrez:   10610
UniGene:   Hs.592105
Chromosome Mapping:   17q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.750000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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