PrimePCR™ SYBR® Green Assay: DGCR2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049553

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DGCR2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DGCR2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042290
Assay Design:   exonic
Chromosome Location:   22:19024810-19024921question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000263196 ENST00000537045 ENST00000545799

Gene Information

Deletions of the 22q11.2 have been associated with a wide range of developmental defects (notably DiGeorge syndrome velocardiofacial syndrome conotruncal anomaly face syndrome and isolated conotruncal cardiac defects) classified under the acronym CATCH 22. The DGCR2 gene encodes a novel putative adhesion receptor protein which could play a role in neural crest cells migration a process which has been proposed to be altered in DiGeorge syndrome. Alternative splicing results in multiple transcript variants.[provided by RefSeq May 2010]

Gene Symbol:   DGCR2
Gene Name:   DiGeorge syndrome critical region gene 2
Aliases:   DGS-C, DKFZp686I1730, IDD, KIAA0163, LAN, SEZ-12
RefSeq:   NC_000022.10 NT_011519.10 NG_021333.1
Ensembl:   ENSG00000070413
Entrez:   9993
Chromosome Mapping:   22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.320000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download