PrimePCR™ SYBR® Green Assay: SRBD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049537

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042274
Assay Design:   exonic
Chromosome Location:   2:45616374-45616507question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000263736 ENST00000535761

Gene Information

Description Not Available

Gene Symbol:   SRBD1
Gene Name:   S1 RNA binding domain 1
Aliases:   FLJ10379
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000068784
Entrez:   55133
UniGene:   Hs.14229
Chromosome Mapping:   2p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.380000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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