PrimePCR™ SYBR® Green Assay: FTSJ1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049534

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042271
Assay Design:   exonic
Chromosome Location:   X:48344269-48344365question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000019019 ENST00000348411 ENST00000456787 ENST00000396894 ENST00000601551 ENST00000597753 ENST00000595294 ENST00000594571

Gene Information

The protein encoded by this gene is a member of the S-adenosylmethionine-binding protein family. It is a nucleolar protein and may be involved in the processing and modification of rRNA. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   FTSJ1
Gene Name:   FtsJ homolog 1 (E. coli)
Aliases:   CDLIV, MRX44, MRX9, SPB1, TRM7
RefSeq:   NC_000023.10 NT_079573.4 NG_008879.1
Ensembl:   ENSG00000068438
Entrez:   24140
UniGene:   Hs.23170
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.650000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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