PrimePCR™ SYBR® Green Assay: IARS2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049524

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042261
Assay Design:   exonic
Chromosome Location:   1:220284170-220287660question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000366922 ENST00000302637

Gene Information

Description Not Available

Gene Symbol:   IARS2
Gene Name:   isoleucyl-tRNA synthetase 2, mitochondrial
Aliases:   FLJ10326, ILERS
RefSeq:   NC_000001.10 NG_015837.1 NT_167186.1
Ensembl:   ENSG00000067704
Entrez:   55699
UniGene:   Hs.262823
Chromosome Mapping:   1q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.520000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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