PrimePCR™ SYBR® Green Assay: KLF6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049512

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042249
Assay Design:   exonic
Chromosome Location:   10:3824111-3824235question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000497571 ENST00000469435 ENST00000542957

Gene Information

This gene encodes a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator and functions as a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene some of which are implicated in carcinogenesis. [provided by RefSeq May 2009]

Gene Symbol:   KLF6
Gene Name:   Kruppel-like factor 6
Aliases:   BCD1, CBA1, COPEB, CPBP, DKFZp686N0199, GBF, PAC1, ST12, ZF9
RefSeq:   NC_000010.10 NT_008705.16 NG_012277.1
Ensembl:   ENSG00000067082
Entrez:   1316
UniGene:   Hs.4055
Chromosome Mapping:   10p15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.460000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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