PrimePCR™ SYBR® Green Assay: RFXANK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049472

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RFXANK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RFXANK, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042209
Assay Design:   exonic
Chromosome Location:   19:19303348-19303885question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000371865 ENST00000421262 ENST00000456252 ENST00000303088 ENST00000593273 ENST00000407360 ENST00000543652 ENST00000540981 ENST00000392324 ENST00000353145

Gene Information

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene along with regulatory factor X-associated protein and regulatory factor-5 forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter this complex associates with the non-DNA-binding factor MHC class II transactivator which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II complementation group B. Two transcript variants encoding different isoforms have been described for this gene with only one isoform showing activation activity. [provided by RefSeq Jul 2008]

Gene Symbol:   RFXANK
Gene Name:   regulatory factor X-associated ankyrin-containing protein
Aliases:   ANKRA1, BLS, F14150_1, MGC138628, RFX-B
RefSeq:   NC_000019.9 NG_007432.1 NT_011295.11
Ensembl:   ENSG00000064490
Entrez:   8625
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.190000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download