This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049472
PrimePCR™ PreAmp for SYBR® Green Assay: RFXANK, Human
PrimePCR™ Template for SYBR® Green Assay: RFXANK, Human
Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene along with regulatory factor X-associated protein and regulatory factor-5 forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter this complex associates with the non-DNA-binding factor MHC class II transactivator which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II complementation group B. Two transcript variants encoding different isoforms have been described for this gene with only one isoform showing activation activity. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.