PrimePCR™ SYBR® Green Assay: WISP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049466

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042203
Assay Design:   exonic
Chromosome Location:   20:43356333-43356452question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000372868 ENST00000372865 ENST00000190983

Gene Information

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain von Willebrand factor type C module thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue and may play an important role in modulating bone turnover. [provided by RefSeq Jul 2008]

Gene Symbol:   WISP2
Gene Name:   WNT1 inducible signaling pathway protein 2
Aliases:   CCN5, CT58, CTGF-L
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000064205
Entrez:   8839
UniGene:   Hs.592145
Chromosome Mapping:   20q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.830000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download