PrimePCR™ SYBR® Green Assay: ZNF275, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049462

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042199
Assay Design:   exonic
Chromosome Location:   X:152613646-152613788question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000509330 ENST00000370251 ENST00000370249 ENST00000421401 ENST00000440091 ENST00000597678 ENST00000593676 ENST00000599931 ENST00000596013

Gene Information

This gene encodes a zinc finger protein that appears to be conserved in eutheria. Its function has not yet been established. [provided by RefSeq Jul 2010]

Gene Symbol:   ZNF275
Gene Name:   zinc finger protein 275
Aliases:   Not Available
RefSeq:   NC_000023.10 NG_021432.1 NT_167198.1
Ensembl:   ENSG00000063587
Entrez:   10838
UniGene:   Hs.348963
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.440000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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