PrimePCR™ SYBR® Green Assay: CCAR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049443

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042180
Assay Design:   exonic
Chromosome Location:   10:70481015-70482323question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000536391 ENST00000265872 ENST00000538031 ENST00000543719 ENST00000539539 ENST00000543225 ENST00000494903 ENST00000535016

Gene Information

Description Not Available

Gene Symbol:   CCAR1
Gene Name:   cell division cycle and apoptosis regulator 1
Aliases:   MGC44628, RP11-437A18.1
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000060339
Entrez:   55749
UniGene:   Hs.49853
Chromosome Mapping:   10q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 37.370000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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