PrimePCR™ SYBR® Green Assay: PHF21B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049423

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042160
Assay Design:   exonic
Chromosome Location:   22:45312333-45312510question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000403565 ENST00000313237 ENST00000420689 ENST00000396103 ENST00000404079 ENST00000447824

Gene Information

Description Not Available

Gene Symbol:   PHF21B
Gene Name:   PHD finger protein 21B
Aliases:   BHC80L, FLJ34161, PHF4
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000056487
Entrez:   112885
UniGene:   Hs.254097
Chromosome Mapping:   22q13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.680000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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