PrimePCR™ SYBR® Green Assay: LETMD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049391

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LETMD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LETMD1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042128
Assay Design:   exonic
Chromosome Location:   12:51442845-51445884question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000551477 ENST00000550929 ENST00000262055 ENST00000550442 ENST00000549340 ENST00000548209 ENST00000548251 ENST00000550814 ENST00000547660 ENST00000548401 ENST00000418425 ENST00000547008 ENST00000547256 ENST00000380123

Gene Information

This gene encodes a mitochondrial outer membrane protein. It has a potential role in tumorigenesis which may result from negative regulation of the p53 tumor suppressor gene. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   LETMD1
Gene Name:   LETM1 domain containing 1
Aliases:   1110019O13Rik, DKFZp586A011, HCCR-1, HCCR-2, HCCR1, HCRR-2
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000050426
Entrez:   25875
Chromosome Mapping:   12q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.960000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download