PrimePCR™ SYBR® Green Assay: RCN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049384

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042121
Assay Design:   exonic
Chromosome Location:   11:32118718-32119905question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000367837 ENST00000530348 ENST00000532942 ENST00000054950 ENST00000532721

Gene Information

Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif the EF-hand. High conservation of amino acid residues outside of these motifs in comparison to mouse reticulocalbin is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.[provided by RefSeq Jan 2009]

Gene Symbol:   RCN1
Gene Name:   reticulocalbin 1, EF-hand calcium binding domain
Aliases:   FLJ37041, FLJ55835, PIG20, RCAL, RCN
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000049449
Entrez:   5954
UniGene:   Hs.97887
Chromosome Mapping:   11p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.550000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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