PrimePCR™ SYBR® Green Assay: CELF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049378

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042115
Assay Design:   exonic
Chromosome Location:   10:11363187-11363283question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000315874 ENST00000354440 ENST00000354897 ENST00000379261 ENST00000416382 ENST00000450189 ENST00000542579 ENST00000399850 ENST00000417956 ENST00000427450 ENST00000537122

Gene Information

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains one C-terminal RRM domain and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   CELF2
Gene Name:   CUGBP, Elav-like family member 2
Aliases:   BRUNOL3, CUGBP2, ETR-3, ETR3, NAPOR
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000048740
Entrez:   10659
UniGene:   Hs.309288
Chromosome Mapping:   10p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.230000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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