PrimePCR™ SYBR® Green Assay: ATP6V1H, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049365

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042102
Assay Design:   exonic
Chromosome Location:   8:54745638-54754247question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000355221 ENST00000359530 ENST00000396774 ENST00000521275 ENST00000524234

Gene Information

This gene encodes a component of vacuolar ATPase (V-ATPase) a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting zymogen activation receptor-mediated endocytosis and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits two G subunits plus the C D E F and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a c c' c" and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit. [provided by RefSeq Jul 2008]

Gene Symbol:   ATP6V1H
Gene Name:   ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
Aliases:   MSTP042, NBP1, SFD, SFDalpha, SFDbeta, VMA13
RefSeq:   NC_000008.10 NT_008183.19
Ensembl:   ENSG00000047249
Entrez:   51606
UniGene:   Hs.491737
Chromosome Mapping:   8q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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