PrimePCR™ SYBR® Green Assay: FBXO42, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049332

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FBXO42, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FBXO42, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042069
Assay Design:   exonic
Chromosome Location:   1:16577885-16578012question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000375592 ENST00000456164 ENST00000444116

Gene Information

Members of the F-box protein family such as FBXO42 are characterized by an approximately 40-amino acid F-box motif. SCF complexes formed by SKP1 (SKP1A; MIM 601434) cullin (see CUL1; MIM 603134) and F-box proteins act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box and they interact with ubiquitination targets through other protein interaction domains (Jin et al. 2004 [PubMed 15520277]).[supplied by OMIM Dec 2010]

Gene Symbol:   FBXO42
Gene Name:   F-box protein 42
Aliases:   Fbx42, JFK, KIAA1332
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000037637
Entrez:   54455
Chromosome Mapping:   1p36.23-p36.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.500000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download