PrimePCR™ SYBR® Green Assay: RPL26L1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049330

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042067
Assay Design:   exonic
Chromosome Location:   5:172385990-172386945question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000519974 ENST00000521476 ENST00000265100 ENST00000519239 ENST00000519522 ENST00000519156 ENST00000583515 ENST00000578812 ENST00000584343 ENST00000583011 ENST00000582556 ENST00000584164 ENST00000293842

Gene Information

This gene encodes a protein that shares high sequence similarity with ribosomal protein L26. It is not currently known whether the encoded protein is a functional ribosomal protein or whether it has evolved a function that is independent of the ribosome. Transcript variants utilizing alternative polyA signals exist. [provided by RefSeq Jul 2008]

Gene Symbol:   RPL26L1
Gene Name:   ribosomal protein L26-like 1
Aliases:   FLJ46904, RPL26P1
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000037241
Entrez:   51121
UniGene:   Hs.546390
Chromosome Mapping:   5q35.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.540000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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