PrimePCR™ SYBR® Green Assay: ZCCHC8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049312

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042049
Assay Design:   exonic
Chromosome Location:   12:122966541-122967194question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000536306 ENST00000543897 ENST00000544054 ENST00000536663 ENST00000540586 ENST00000336229

Gene Information

Description Not Available

Gene Symbol:   ZCCHC8
Gene Name:   zinc finger, CCHC domain containing 8
Aliases:   DKFZp434E2220
RefSeq:   NC_000012.11 NT_009755.19
Ensembl:   ENSG00000033030
Entrez:   55596
UniGene:   Hs.37706
Chromosome Mapping:   12q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.740000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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