PrimePCR™ SYBR® Green Assay: ARNTL2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049306

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042043
Assay Design:   exonic
Chromosome Location:   12:27553483-27553624question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000544915 ENST00000395901 ENST00000546179 ENST00000311001 ENST00000261178 ENST00000266503 ENST00000457040 ENST00000542388

Gene Information

This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (PER ARNT SIM) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels exposure to certain environmental pollutants and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian CLOCK protein the structurally related MOP4 and hypoxia-inducible factors such as HIF1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors this protein is coexpressed in regions of the brain such as the thalamus hypothalamus and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   ARNTL2
Gene Name:   aryl hydrocarbon receptor nuclear translocator-like 2
Aliases:   BMAL2, CLIF, MGC149671, MGC149672, MOP9, PASD9, bHLHe6
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000029153
Entrez:   56938
UniGene:   Hs.445447
Chromosome Mapping:   12p12.2-p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.620000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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