This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0049297
PrimePCR™ PreAmp for SYBR® Green Assay: VIM, Human
PrimePCR™ Template for SYBR® Green Assay: VIM, Human
This gene encodes a member of the intermediate filament family. Intermediate filamentents along with microtubules and actin microfilaments make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape integrity of the cytoplasm and stabilizing cytoskeletal interactions. It is also involved in the immune response and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment migration and cell signaling. Mutations in this gene causes a dominant pulverulent cataract.[provided by RefSeq Jun 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.