PrimePCR™ SYBR® Green Assay: RNF10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049283

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042020
Assay Design:   exonic
Chromosome Location:   12:121001739-121002987question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000325954 ENST00000413266 ENST00000546262 ENST00000540046

Gene Information

The protein encoded by this gene contains a ring finger motif which is known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. EST data suggests the existence of multiple alternatively spliced transcript variants however their full length nature is not known. [provided by RefSeq Jul 2008]

Gene Symbol:   RNF10
Gene Name:   ring finger protein 10
Aliases:   KIAA0262, MGC126758, MGC126764, RIE2
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000022840
Entrez:   9921
UniGene:   Hs.442798
Chromosome Mapping:   12q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.640000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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