PrimePCR™ SYBR® Green Assay: PRDM11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049276

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042013
Assay Design:   exonic
Chromosome Location:   11:45203812-45204425question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000263765 ENST00000534751 ENST00000424263 ENST00000526442 ENST00000530656

Gene Information

Description Not Available

Gene Symbol:   PRDM11
Gene Name:   PR domain containing 11
Aliases:   PFM8
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000019485
Entrez:   56981
UniGene:   Hs.178715
Chromosome Mapping:   11p11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.320000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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