This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049268
PrimePCR™ PreAmp for SYBR® Green Assay: SLC38A5, Human
PrimePCR™ Template for SYBR® Green Assay: SLC38A5, Human
The protein encoded by this gene is a system N sodium-coupled amino acid transporter involved in the transfer of glutamine asparagine histidine serine alanine and glycine. The encoded protein does not transport charged amino acids imino acids or N-alkylated amino acids. This transporter is not inhibited by lithium.[provided by RefSeq Jun 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.