This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0049262
PrimePCR™ PreAmp for SYBR® Green Assay: NUDCD3, Human
PrimePCR™ Template for SYBR® Green Assay: NUDCD3, Human
The product of this gene functions to maintain the stability of dynein intermediate chain. Depletion of this gene product results in aggregation and degradation of dynein intermediate chain mislocalization of the dynein complex from kinetochores spindle microtubules and spindle poles and loss of gamma-tubulin from spindle poles. The protein localizes to the Golgi apparatus during interphase and levels of the protein increase after the G1/S transition. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.