PrimePCR™ SYBR® Green Assay: LRRC23, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049226

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LRRC23, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LRRC23, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041963
Assay Design:   exonic
Chromosome Location:   12:7014790-7014889question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000321037 ENST00000533871 ENST00000321016 ENST00000433346 ENST00000007969 ENST00000323702 ENST00000443597 ENST00000415834 ENST00000436789 ENST00000429740 ENST00000263257 ENST00000242848 ENST00000282007 ENST00000308251 ENST00000296575 ENST00000434550 ENST00000601429 ENST00000598122 ENST00000600515 ENST00000599171 ENST00000595209 ENST00000597585 ENST00000600963

Gene Information

Description Not Available

Gene Symbol:   LRRC23
Gene Name:   leucine rich repeat containing 23
Aliases:   LRPB7
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000010626
Entrez:   10233
Chromosome Mapping:   12p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.280000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download