PrimePCR™ SYBR® Green Assay: RAD52, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049135

List Price:    $146.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041872
Assay Design:   exonic
Chromosome Location:   12:1040473-1042227question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000358495 ENST00000430095 ENST00000536177 ENST00000542785 ENST00000397230 ENST00000544742 ENST00000541619 ENST00000545564

Gene Information

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52 a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51 which suggested its role in RAD51 related DNA recombination and repair. [provided by RefSeq Jul 2008]

Gene Symbol:   RAD52
Gene Name:   RAD52 homolog (S. cerevisiae)
Aliases:   Not Available
RefSeq:   NC_000012.11 NG_007984.2 NG_017078.1 NT_009759.16
Ensembl:   ENSG00000002016
Entrez:   5893
UniGene:   Hs.410355
Chromosome Mapping:   12p13-p12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 35.990000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download