PrimePCR™ SYBR® Green Assay: RP11-1286E23.17, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041843

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RP11-1286E23.17, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RP11-1286E23.17, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041774
Assay Design:   exonic
Chromosome Location:   4:9269970-9270103question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000515566 ENST00000506151 ENST00000506414 ENST00000511280 ENST00000504481 ENST00000509271 ENST00000509660 ENST00000507227 ENST00000515574 ENST00000504543 ENST00000511681 ENST00000504104 ENST00000333796 ENST00000429667 ENST00000421288 ENST00000417945 ENST00000457736 ENST00000415041 ENST00000456464 ENST00000511568 ENST00000504209

Gene Information

Description Not Available

Gene Symbol:   RP11-1286E23.17
Gene Name:   ubiquitin carboxyl-terminal hydrolase 17
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000248933
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 33.690000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download