PrimePCR™ SYBR® Green Assay: SCXA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041824

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041755
Assay Design:   exonic
Chromosome Location:   8:145490901-145490990question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000347230 ENST00000295550 ENST00000472056 ENST00000409809 ENST00000347401 ENST00000353578 ENST00000346358 ENST00000407558 ENST00000340210 ENST00000340695 ENST00000407315 ENST00000252999 ENST00000246080 ENST00000325509 ENST00000366787

Gene Information

Description Not Available

Gene Symbol:   SCXA
Gene Name:   scleraxis homolog A (mouse)
Aliases:   SCX, SCXB, bHLHa41
RefSeq:   NC_000008.10 NT_037704.5
Ensembl:   ENSG00000188686
Entrez:   100129885
UniGene:   Hs.723088
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.160000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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