PrimePCR™ SYBR® Green Assay: SERF1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041727

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SERF1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SERF1B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0039005
Assay Design:   exonic
Chromosome Location:   5:69322241-69337354question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000576191 ENST00000572408 ENST00000573686 ENST00000572521 ENST00000573393 ENST00000573188 ENST00000573668 ENST00000570647 ENST00000392474 ENST00000545538 ENST00000380751 ENST00000380750 ENST00000503931 ENST00000506542 ENST00000515588 ENST00000317633 ENST00000511162 ENST00000354833 ENST00000513436 ENST00000504458 ENST00000507348 ENST00000512868 ENST00000512649 ENST00000377086 ENST00000377081 ENST00000287139

Gene Information

This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is the centromeric copy which is identical to the telomeric copy. Alternatively spliced transcripts have been documented but it is unclear whether alternative splicing occurs for both the centromeric and telomeric copies of the gene. The gene encodes a protein of unknown function which bears low-level homology with the RNA-binding domain of matrin-cyclophilin a protein which colocalizes with small nuclear ribonucleoproteins (snRNPs) and the SMN1 gene product. [provided by RefSeq Jul 2008]

Gene Symbol:   SERF1B
Gene Name:   small EDRK-rich factor 1B (centromeric)
Aliases:   FAM2B, H4F5C, MGC125278, MGC125279, MGC125280, SERF1A
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000205572
Entrez:   728492
Chromosome Mapping:   5q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.420000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download