PrimePCR™ SYBR® Green Assay: VCX2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041679

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038957
Assay Design:   exonic
Chromosome Location:   X:8138303-8138601question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000297261 ENST00000305869 ENST00000250825 ENST00000250823 ENST00000405109 ENST00000407609 ENST00000343941 ENST00000405256 ENST00000381089 ENST00000398729 ENST00000381059 ENST00000341408 ENST00000317103 ENST00000381032 ENST00000453306 ENST00000444481 ENST00000440654 ENST00000381029 ENST00000268876 ENST00000394570 ENST00000433649 ENST00000378449 ENST00000564134 ENST00000568666 ENST00000261448 ENST00000456138 ENST00000262623 ENST00000369519

Gene Information

This gene belongs to the VCX/Y gene family which has multiple members on both X and Y chromosomes and all are expressed exclusively in male germ cells. The X-linked members are clustered on chromosome Xp22 and Y-linked members are two identical copies of the gene within a palindromic region on Yq11. The family members share a high degree of sequence identity with the exception that a 30-bp unit is tandemly repeated in X-linked members but occurs only once in Y-linked members. The VCX gene cluster is polymorphic in terms of copy number; different individuals may have a different number of VCX genes. VCX/Y genes encode small and highly charged proteins of unknown function. The presence of a putative bipartite nuclear localization signal suggests that VCX/Y members are nuclear proteins. This gene contains 2 repeats of the 30-bp unit. [provided by RefSeq Jul 2008]

Gene Symbol:   VCX2
Gene Name:   variable charge, X-linked 2
Aliases:   MGC118977, MGC125729, MGC125794, MGC125795, VCX-2r, VCX2R, VCXB
RefSeq:   NC_000023.10 NT_167197.1
Ensembl:   ENSG00000177504
Entrez:   51480
UniGene:   Hs.279737
Chromosome Mapping:   Xp22.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996400
y-intercept 36.370000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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