PrimePCR™ SYBR® Green Assay: RTBDN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041649

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RTBDN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RTBDN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038927
Assay Design:   exonic
Chromosome Location:   19:12940625-12940751question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000381020 ENST00000404898 ENST00000402142 ENST00000336649 ENST00000400164 ENST00000405493 ENST00000546530 ENST00000261729 ENST00000446861 ENST00000548055 ENST00000300119 ENST00000442789 ENST00000544473 ENST00000527670 ENST00000534444 ENST00000458235 ENST00000312143 ENST00000412676 ENST00000397688 ENST00000479991 ENST00000545866 ENST00000333139 ENST00000446232 ENST00000278505 ENST00000279178 ENST00000310969 ENST00000377266 ENST00000454679 ENST00000549114 ENST00000341413 ENST00000564537 ENST

Gene Information

Description Not Available

Gene Symbol:   RTBDN
Gene Name:   retbindin
Aliases:   FLJ36353
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000132026
Entrez:   83546
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.660000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download