PrimePCR™ SYBR® Green Assay: C20orf30, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041612

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038890
Assay Design:   exonic
Chromosome Location:   20:5081003-5081133question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000379277 ENST00000379299 ENST00000379286 ENST00000379279 ENST00000379283 ENST00000342308 ENST00000202834

Gene Information

Description Not Available

Gene Symbol:   C20orf30
Gene Name:   chromosome 20 open reading frame 30
Aliases:   HSPC274, dJ1116H23.2.1
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000089063
Entrez:   29058
UniGene:   Hs.472024
Chromosome Mapping:   20p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 37.260000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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