PrimePCR™ SYBR® Green Assay: TAS2R30, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041560

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TAS2R30, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TAS2R30, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038838
Assay Design:   exonic
Chromosome Location:   12:11286440-11286589question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000570541 ENST00000574336 ENST00000575161 ENST00000573885 ENST00000571012 ENST00000506868 ENST00000390673 ENST00000390675 ENST00000533467 ENST00000531678 ENST00000539585 ENST00000575654 ENST00000573562 ENST00000572376 ENST00000574263 ENST00000571879 ENST00000576640 ENST00000602487 ENST00000602542

Gene Information

Description Not Available

Gene Symbol:   TAS2R30
Gene Name:   taste receptor, type 2, member 30
Aliases:   T2R30, T2R47, TAS2R47
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000256188
Entrez:   259293
Chromosome Mapping:   12p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.310000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download