PrimePCR™ SYBR® Green Assay: RP13-395E19.1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041556

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RP13-395E19.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RP13-395E19.1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038834
Assay Design:   exonic
Chromosome Location:   15:32685259-32685376question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000563027 ENST00000567927 ENST00000341650 ENST00000569052 ENST00000327271 ENST00000562783 ENST00000566740 ENST00000587283 ENST00000512626 ENST00000509311 ENST00000569659 ENST00000448387 ENST00000450802 ENST00000562295 ENST00000358107 ENST00000409737 ENST00000393972 ENST00000233615

Gene Information

Description Not Available

Gene Symbol:   RP13-395E19.1
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000249931
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 37.060000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download