PrimePCR™ SYBR® Green Assay: TCEAL6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041519

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TCEAL6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TCEAL6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038797
Assay Design:   exonic
Chromosome Location:   X:101396073-101396170question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000289175 ENST00000244815 ENST00000392000 ENST00000409204 ENST00000409186 ENST00000355413 ENST00000473247 ENST00000535825 ENST00000414043 ENST00000361571 ENST00000572543 ENST00000381311 ENST00000348066 ENST00000358183 ENST00000217073 ENST00000255136 ENST00000011292 ENST00000598830 ENST00000372774 ENST00000372773 ENST00000372680 ENST00000372628 ENST00000372627 ENST00000243286 ENST00000301015 ENST00000579597 ENST00000598476 ENST00000597954 ENST00000575872 ENST00000573706 ENST00000586755

Gene Information

Description Not Available

Gene Symbol:   TCEAL6
Gene Name:   transcription elongation factor A (SII)-like 6
Aliases:   Tceal3
RefSeq:   NC_000023.10 NT_011651.17
Ensembl:   ENSG00000204071
Entrez:   158931
Chromosome Mapping:   Xq22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.780000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download