PrimePCR™ SYBR® Green Assay: ZNF493, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041506

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF493, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF493, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038784
Assay Design:   exonic
Chromosome Location:   19:21579947-21580044question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000448023 ENST00000359128 ENST00000301749 ENST00000419350 ENST00000360117 ENST00000596302 ENST00000392288 ENST00000594390 ENST00000355504 ENST00000339914 ENST00000313732 ENST00000322734 ENST00000342679 ENST00000361818 ENST00000558239

Gene Information

Description Not Available

Gene Symbol:   ZNF493
Gene Name:   zinc finger protein 493
Aliases:   FLJ36504
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000196268
Entrez:   284443
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.800000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download