PrimePCR™ SYBR® Green Assay: IFITM2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041494

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IFITM2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IFITM2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038772
Assay Design:   exonic
Chromosome Location:   11:309115-309213question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000602569 ENST00000399817 ENST00000533141 ENST00000602735 ENST00000526811 ENST00000399808 ENST00000401642 ENST00000391860 ENST00000525115 ENST00000321327 ENST00000597505

Gene Information

Description Not Available

Gene Symbol:   IFITM2
Gene Name:   interferon induced transmembrane protein 2 (1-8D)
Aliases:   1-8D
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000185201
Entrez:   10581
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.590000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download