PrimePCR™ SYBR® Green Assay: HYI, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041486

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HYI, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HYI, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038764
Assay Design:   exonic
Chromosome Location:   1:43917890-43917990question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000372442 ENST00000562955 ENST00000372426 ENST00000583037 ENST00000372425 ENST00000486909 ENST00000372432 ENST00000372430 ENST00000487366 ENST00000470662 ENST00000372434 ENST00000372433 ENST00000425673 ENST00000359651 ENST00000298351 ENST00000375400 ENST00000355867 ENST00000375398 ENST00000535393 ENST00000419488 ENST00000354212 ENST00000522391 ENST00000535697 ENST00000536571 ENST00000542569

Gene Information

This gene encodes a putative hydroxypyruvate isomerase which likely catalyzes the conversion of hydroxypyruvate to 2-hydroxy-3-oxopropanoate and may be involved in carbohydrate transport and metabolism. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   HYI
Gene Name:   hydroxypyruvate isomerase (putative)
Aliases:   HT036, MGC20767
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000178922
Entrez:   81888
Chromosome Mapping:   1p34.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.310000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download