PrimePCR™ SYBR® Green Assay: ZNF417, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041476

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF417, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF417, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038754
Assay Design:   exonic
Chromosome Location:   19:58423438-58423530question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000600053 ENST00000536878 ENST00000597850 ENST00000598689 ENST00000599456 ENST00000594684 ENST00000512439 ENST00000426889 ENST00000317178 ENST00000431353 ENST00000451849 ENST00000594473 ENST00000391701 ENST00000597134 ENST00000596498 ENST00000598031 ENST00000594328 ENST00000594901 ENST00000442832 ENST00000316462 ENST00000596604 ENST00000597342 ENST00000597832 ENST00000600634 ENST00000595048 ENST00000435989 ENST00000597807 ENST00000423137 ENST00000339656 ENST00000419854 ENST00000594396 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF417
Gene Name:   zinc finger protein 417
Aliases:   MGC34079
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000173480
Entrez:   147687
Chromosome Mapping:   19q13.43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.900000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download