PrimePCR™ SYBR® Green Assay: POMZP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041434

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: POMZP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: POMZP3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038712
Assay Design:   exonic
Chromosome Location:   7:76254887-76254987question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000395270 ENST00000358357 ENST00000434423 ENST00000446813 ENST00000257622 ENST00000257665 ENST00000453279 ENST00000275569 ENST00000310842 ENST00000454397 ENST00000575107 ENST00000575723 ENST00000575645 ENST00000574731 ENST00000573728 ENST00000576503

Gene Information

This gene appears to have resulted from a fusion of DNA sequences derived from 2 distinct loci specifically through the duplication of two internal exons from the POM121 gene and four 3' exons from the ZP3 gene. The 5' end of this gene is similar to the 5` coding region of the POM121 gene which encodes an integral nuclear pore membrane protein. However the protein encoded by this gene lacks the nuclear pore localization motif. The 3' end of this gene is similar to the last 4 exons of the zona pellucida glycoprotein 3 (ZP3) gene and the encoded protein retains one zona pellucida domain. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   POMZP3
Gene Name:   POM121 and ZP3 fusion
Aliases:   MGC8359, POM-ZP3, POM121
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000146707
Entrez:   22932
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 35.590000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download