PrimePCR™ SYBR® Green Assay: DCAF8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041419

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DCAF8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DCAF8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038697
Assay Design:   exonic
Chromosome Location:   1:160210076-160213763question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000441525 ENST00000506447 ENST00000511820 ENST00000430049 ENST00000325971 ENST00000368073 ENST00000326837 ENST00000368074 ENST00000447377 ENST00000440682 ENST00000407642 ENST00000419626 ENST00000555195 ENST00000556710 ENST00000485079

Gene Information

This gene encodes a WD repeat-containing protein that interacts with the Cul4-Ddb1 E3 ligase macromolecular complex. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Jul 2009]

Gene Symbol:   DCAF8
Gene Name:   DDB1 and CUL4 associated factor 8
Aliases:   DKFZp781G1096, FLJ35857, H326, MGC117276, MGC118891, MGC99640, WDR42A
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000132716
Entrez:   50717
Chromosome Mapping:   1q22-q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.730000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download