PrimePCR™ SYBR® Green Assay: TIMM17B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041413

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TIMM17B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TIMM17B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038691
Assay Design:   exonic
Chromosome Location:   X:48751403-48751500question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000376582 ENST00000396779 ENST00000367287 ENST00000596806 ENST00000593291

Gene Information

This gene encodes a protein that is part of the mitochondrial transport TIMM23 complex. TIM translocase complexes facilitate the transport of mitochondrial proteins from the cytosol across the mitochondrial inner membrane and into the mitochondrion. Two alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq Nov 2009]

Gene Symbol:   TIMM17B
Gene Name:   translocase of inner mitochondrial membrane 17 homolog B (yeast)
Aliases:   DXS9822, TIM17B
RefSeq:   NC_000023.10 NG_015967.1 NT_079573.4 NG_015968.1
Ensembl:   ENSG00000126768
Entrez:   10245
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.680000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download