PrimePCR™ SYBR® Green Assay: NPDC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041391

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: NPDC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NPDC1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038669
Assay Design:   exonic
Chromosome Location:   9:139935292-139935436question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000562641 ENST00000398841 ENST00000170150 ENST00000318222 ENST00000338519 ENST00000403437 ENST00000319023 ENST00000530055 ENST00000539574 ENST00000402050 ENST00000404984 ENST00000583535 ENST00000226209 ENST00000336418 ENST00000376355 ENST00000371600 ENST00000371601 ENST00000373109 ENST00000317376 ENST00000536168 ENST00000409816 ENST00000409090 ENST00000389524 ENST00000428314 ENST00000528896 ENST00000389003 ENST00000544884 ENST00000389441 ENST00000265109 ENST00000512629 ENST00000428746 ENST

Gene Information

Description Not Available

Gene Symbol:   NPDC1
Gene Name:   neural proliferation, differentiation and control, 1
Aliases:   CAB, CAB-, CAB-1, CAB1, DKFZp586J0523, NPDC-1
RefSeq:   NC_000009.11 NT_024000.16
Ensembl:   ENSG00000107281
Entrez:   56654
Chromosome Mapping:   9q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.992900
y-intercept 35.650000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download